pBOOST2 saIRF7/3
| Product | Unit size | Cat. code | Docs. | Price | |
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pBOOST2-sahIRF7/3 pBOOST2 Human Chimeric IRF7/3 |
Show product |
20 µg |
pbst2-sahirf73
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pBOOST2-samIRF7/3 pBOOST2 Murine Chimeric IRF7/3 |
Show product |
20 µg |
pbst2-samirf73
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Genetic adjuvants for DNA vaccines with chimeric IRF-7/IRF-3 gene | Increase both Th1 T-cell and Th2 responses
pBOOST2 saIRF7/3 plasmids express a human or murine super-activated IRF7/3 chimeric gene.
IRF-3 and IRF-7 increase both Th1 T-cell and Th2 antibody responses by transactivating different target promoters [1].
To exploit the biological features of both IRFs, a chimeric form of IRF-7 and IRF-3 was generated by combining the DNA binding specificity of IRF-7 with the strong transactivation capacity of super-activated IRF-3.
IRF-7/3 chimera provides >10-fold greater induction of IFN-α and IFN-β promoters than super-activated IRF-3 alone [2].
pBOOST2-saIRF3 plasmids express the transgene under the control of the strong and ubiquitous EF-1α/HTLV composite promoter and are selectable in E. coli with Zeocin™.
References:
1. Sasaki S. et al., 2002. Regulation of DNA-raised immune responses by cotransfected interferon regulatory factors. J Virol.76(13):6652-9.
2. Bramson JL. et al., 2003. Super-activated interferon-regulatory factors can enhance plasmid immunization. Vaccine. 21(13-14):1363-70.
Specifications
Gene: super-activated chimeric IRF-7 / IRF-3 gene
Species: Human or Murine
Backbone: pBOOST2
Selection: Zeocin
Th1 and Th2 responses
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- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
